Unpublished report · Livestock · In the living system

A follow-up dairy trial found no change in feed efficiency or milk, with shifts in milk fatty acids.

  • Unpublished report
  • Dose unconfirmed

In this unpublished follow-up, feed efficiency, milk yield, fat, protein and linear score did not change at six weeks, and which of two printed doses was delivered is not yet confirmed.

University of Guelph, Ridgetown Campus, 2024. Final results report. Manufacturer-supplied report.

This report is not published. Enquiries about it, including access to the report where its owners permit, can be sent to info@carbonbiomeproject.com.

Scope, material and route

Studied
Lactating dairy cows
Design
Two parallel groups, balanced for days in milk, lactation, projected yield and pregnancy status; not a crossover
Setting
University of Guelph, Ridgetown Campus dairy unit, Ontario
Material
A humic and fulvic preparation supplied by its manufacturer
Route
Drinking water
Exposure
The report prints 0.05 mL per litre of water; the trial proposal states 0.5 mL per litre. The two differ tenfold, and which was delivered is unconfirmed; the project has asked the authors. Study exposure, not application guidance.
Duration
Six weeks

Study design

  1. The dose

    • The report prints0.05 mL per litre of water
    • The trial proposal states0.5 mL per litre
    • DeliveredUnconfirmed; the project has asked the authors
  2. The cows

    Two parallel groups, started two weeks apart; six weeks on treated or untreated water.

    24 cows12 per group.

  3. No difference at six weeks

    • Feed efficiency1.00 against 1.02 kg ECM per kg dry matter
    • Milk yield39.8 against 40.3 kg
    • Fat and protein4.0 against 3.9; 3.3 against 3.3 percent
    • Somatic cell linear score3.8 against 3.8
  4. What differed

    • Myristic and medium-chain fatty acidsHigher
    • Rumination, week 3507 against 464 minutes a day; not at week 6
    • Feed sorting, manure particle sizeTendencies
Values are treated group first, untreated second, at week 6 unless stated. The authors say some effects could reflect how the two groups were formed. The report is unpublished and has not been peer reviewed.

More study details

Research question
Does the preparation in drinking water change feed efficiency, milk production, milk composition or behaviour?
Sample size
24 cows, 12 per group
Comparator
Untreated drinking water
Outcomes measured
Feed efficiency, milk yield and components, milk fatty acid profile, somatic cell linear score, rumination and leisure time, feed sorting, manure particle size, body temperature
Not measured
Rumen pH and gas in the animal; a manure methane assay was planned and is not reported

Findings

In a follow-up trial at the University of Guelph's Ridgetown Campus, 24 lactating cows received treated or untreated drinking water for six weeks, in two groups of 12 started two weeks apart. At six weeks there was no difference in feed efficiency, milk yield, energy corrected milk, fat, protein or somatic cell linear score. Myristic acid and medium-chain fatty acids in milk were higher. Rumination was 43 minutes a day longer at week 3, which did not persist to week 6. The authors noted that some effects could reflect how the groups were formed. The report is unpublished and has not been peer reviewed.

OutcomeComparison or resultInterpretation
Feed efficiency (kg ECM per kg dry matter)1.00 against 1.02 (P = 0.79)No difference
Milk yield39.8 against 40.3 kg (P = 0.81)No difference
Fat and protein4.0 against 3.9 percent (P = 0.26); 3.3 against 3.3 percent (P = 0.64)No difference
Somatic cell linear score3.8 against 3.8 (P = 0.97)No difference in this trial
Myristic acid (C14:0) and medium-chain fatty acids0.38 against 0.36 (P = 0.03); 1.69 against 1.56 (P = 0.04)Higher with the preparation
Rumination, week 3 and week 6507 against 464 minutes a day at week 3 (significant); 508 against 490 at week 6 (P = 0.34)A transient difference
Feed sorting and manure particle sizeTendencies (P = 0.08 and 0.09)Not statistically supported

Values are treated group first, control second, at week 6 unless stated, as reported. Values are extracted from the source as reported; no raw data were available.

Interpretation and limits

What this record supports

No change in feed efficiency, milk yield, milk fat and protein or somatic cell linear score at six weeks, with small shifts in milk fatty acid composition, for this preparation in this trial.

What remains uncertain

  • Status. The report has not been published or peer reviewed.
  • Dose reporting. The report prints 0.05 mL per litre and the proposal states 0.5 mL per litre; which was delivered is unconfirmed, pending the authors.
  • Design. Two small groups started two weeks apart; the authors say some effects could reflect group creation.

Which part of the framework it informs

  • Environmental conditions

    The physical, chemical and management context.

  • Microbial activity

    What microbial communities do within that context.

  • Observable function

    What can be observed or measured in the host or wider system.

    This record informs this area.
Informs evaluation methods and observable function. A null result on feed efficiency sits beside the earlier trial's lower linear score, and both are needed to read the evidence fairly.

Source, funding and review

Source type
Unpublished report
Associated products
The tested preparation comes from the manufacturer that produces the formulations sold by Fulgenix Farms. Equivalence to a specific Fulgenix Farms product is not documented in this record.
Funding and relationships
Not stated in the results document. The report was provided by the manufacturer of the tested preparation.
Review status
Prepared as an editorial summary. External scientific review has not been completed.
What was read
Read in full from the report (14 pages, including model tables). The report is not publicly available.
Original title
Effect of humate substances on feed efficiency and milk production components of lactating dairy cattle
Authors
University of Guelph, Ridgetown Campus dairy research team
Publication
Final results report, 2024
Summary prepared by
Carbon Biome Project
Date added
19 October 2026
Cite this summary
Carbon Biome Project. A follow-up dairy trial found no change in feed efficiency or milk, with shifts in milk fatty acids. Evidence library record, added 19 October 2026. carbonbiomeproject.com/evidence/guelph-ridgetown-2024-dairy-feed-efficiency/
Updates
2026-10-22 · Study exposure restated as an unresolved discrepancy between the report (0.05 mL per litre) and the trial proposal (0.5 mL per litre). · The earlier entry resolved it as a decimal error without confirmation from the authors.

Related records

The waiting list

Join the waiting list

Leave your email and we will send it to you when it is ready.

One email when your edition is ready. Nothing else.